R., Miguel ÁngelGiraldo, JulianaDurango, Juan CarlosMolina Palacios, DanielAbreu Vélez, Adrián ManuelMoncada Velásquez, MarcelaRamón E, Josué Nicolás2014-10-222014-10-222014-10-222256-3342http://hdl.handle.net/10567/1190Introduction. At the end of milking of the bovine female, the teat sphincter remains open and expanded for about 30 to 60 minutes. This time favors the entry of environmental microorganisms into the mammary gland, causing new infections and compromising the health of the cow. Objective. Bactericidal disinfectants used, such as iodine to prevent entry of microorganisms into the mammary gland after the milking routine, can easily be inactivated by contact with milk, manure, mud and urine; the nipple then remain unprotected and exposed to the entry of different microorganisms. The protective effect of artificial barrier sealant in dipping post-milking routine prevents the contact of the nipple with environmental microorganisms. Materials and method. The effect of a teat dip artificial barrier sealant was evaluated on the post;dipping in a mechanic milking routine preventing the contact between microorganisms and the nipple after the milking. Also the sealant forms a film on the nipple preventing the entry of bacteria into the udder. Two groups of animals (25 per group) were selected, a group was treated with the artificial barrier dipping in the post-milking period and in the control group was perform the normal routine process. In each group the hygienic quality was tested by Colony Forming Units (CFU/mL). Randomness, independence and normality of experimental errors, homogeneity of variance associated with the effects of variables, independence of action and variances: attached to the model assumptions were validated. There was perform descriptive statistics and ANOVA using SAS 9.0 software. Results. It was demonstrated that de use of a barrier protector decreases the amount of bacteria on the nipple surface in the moment of the milking routine.esCorporación Universitaria LasallistaFacultad de Ciencias Administrativas y AgropecuariasNorte AntioqueñoOrdeño mecánicoGanado lecheroHigiene de la lecheArticle