Gutiérrez Ramírez, Luz AdrianaPérez Bran, Juan AlejandroUribe Londoño, Mario Alberto2012-08-212012-08-212012-08-212256-3342http://hdl.handle.net/10567/644Introduction: Cellulose is the most common polysaccharide in nature. The organisms able to degrade it are mainly fungi and bacteria, known for their ability to produce not only cellulose enzymes, but also amylases, proteases and peptidases, among others, which allow them to recycle the organic material back to the soil. Cellulase hydrolysis takes place by an enzyme complex called cellulases, basically made up by three enzymes: endoglucanase, exoglucanase and β-glucosidases. Objective: Given their importance in industry, in this research it is proposed a characterization of native strains of fungi isolated from several substrates, which were determined as Aspergillus niger, Cladosporiumherbarum and Trichoderma reesei, all of them with cellulolytic activity. Materials and methods: This activity was determined by measuring the production of β-exoglucanase, β-endoglucanase and β-glucosidase. Results: Cladosporiumherbarum had the highest β-exoglucanase level, 0.0554 UI/ ml; Aspergillus niger demonstrated a high β-endoglucanase´s production, 0.02463UI/ ml, and Trichoderma reesei had the best β-glucosidase activity, with 0.000138UI/ml. The latter is the fungus with the best cellulolytic activity.esCorporación Universitaria LasallistaFacultad de Ciencias Administrativas y AgropecuariasCelulosaActividad celulolíticaTrichoderma ReeseiCladosporium HerbarumAspergillus NigerHongosInvitroEndoglucanasaExoglucanasaβ-GlucosidasaArticle