Browsing by Author "Solarte Portilla, Carlos Eugenio"
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Item Efecto del tamaño de camada y número de parto en el crecimiento de cuyes(Cavia porcell us Rodentia: caviidae)(Corporación Universitaria Lasallista, 2010) Burgos Paz, William Orlando; Solarte Portilla, Carlos Eugenio; Cerón Muñoz, Mario FernandoGuinea pig is a species with a great potential to produce animal origin protein, given its precocity and fast growth, but just a few reports evaluate the growth of this species and its reproductive characteristics. One way to describe the growth of Guinea pigs is by the use of mathematic non-lineal models, previously used to study many other species, given their easy calculations and data adjustment. Objective. To describe the growth curve of female Guinea pigs by the use of a non linear model and evaluate the correlation of the breed´s size and the delivery number on the parameters of the curves, weight and maturity at different ages. Materials and methods. 2505 weight records of 501 female Guinea pigs were analyzed in the delivery and the weaning, at four, eight and twelve weeks of age. The curve was taken by the use of a Von Bertalanffy´s non linear model. The significance of the independent variables was determined, maturity at 90 (M90) and 120 days (M120), and the weight at 15 (P15), 30 (P30), 60 (P60) and 90 days (P90) by the use of a mixed model that included the effects of the breeds´ size, delivery number, contemporary group and common environment. Results. The variance analysis determined that there is a correlation between the breed’s size and the delivery number for the β 1 , M90, M120, P15, P30 P60 y P90 variables; while there was no correlation for the weight at 120 (P120) and 180 days (P180), and the animals had a similar growth. Conclusions. Von Bertalanffy´s non linear model was useful to adjust the weights of the female Guinea pigs and the modeling of the growth of that species. On the other hand, it was evidenced that a higher number of deliveries brought a faster maturity of young Guinea pigsItem Identificación de polimorfismos del gen de la Kappa caseína bovina: Nariño-Colombia(Corporación Universitaria Lasallista, 2009) Solarte Portilla, Carlos Eugenio; Rosero Galindo, Carol Yovanna; Cárdenas Henao, Heiber; Burgos Paz, William Orlando; Eraso Cabrera, Johanna Melissa; Zambrano Burbano, Gema LucíaIntroduction. The use of cards to collect, store and conserve blood is not enough in molecular biology research, in which these processes are preferably done with Vacutainer tubes with ethylene diamine tetra-acetic acid as an anti coagulant. However, due to the topography of the zone in which such research takes place, the use of conventional methods, with refrigerated transportation of the samples, is not adequate because of the difficult access conditions in the farms and the long distance between herds. Objective. To standardize the technique (chain reaction of simple chain polymerase-polymorphisms) for the identification of polymorphisms of the kappacasein gen in Holstein, Norman, Jersey and Brown Suiss cattle in the dairy basin of the Nariño´s high tropical zone. Material and methods. Blood samples were taken from 1087 animals, using cards for collecting, storing and keeping the tissues for their analysis in the laboratory. Results. To obtain high quality amplified products, one 1.23 mm disc and an electrophoretic time of 16 hours at 160 V were sufficient. This research work allows to conclude that cards were an efficient mean, because they make transportation and storing of blood tissue easy and avoid the use of refrigeration to keep the samples during long periods of time.