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Optimization of an analytical approach based on high-performance liquid chromatography for the characterization of a natural vanilla extract
(Corporación Universitaria Lasallista, 2016) Velez Suaza, Catalina
An overview of flavour industry and specifically vanilla flavour has been realised to understand its importance. Vanilla, as the second most expensive spice in the world, has an important value for flavour industries, but currently falsifications are a big problem, so regulations are increasingly stringent. To this fact, a chromatographic method has been developed to the characterisation of natural vanilla extracts. The method development included a standardisation of the extraction method and a validation of. An extraction method of a natural vanilla extract has been developed, as part of the analytical method, using an emergent technology called Ultrasonic Assisted Extraction (UAE). This study has enabled to probe the role of UAE in enhancing the evaluated responses by a comparison study with a conventional extraction. A fast, precise and accurate RP-HPLC method has been developed for the working conditions of Sevarome laboratory, by the stabilised regulations of method standardization of ICH. The method was validated for linearity, selectivity, LOD, LOQ, precision and accuracy. The method is useful for the identification and quantification of the main compounds in natural vanilla extracts and other vanilla products.