Browsing by Author "Franco Anaya, Piedad Astrith"
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Item Determinación de Escherichia Coli e identificación del serotipo O157:H7 en carne de cerdo comercializada en los principales supermercados de la ciudad de Cartagena(Corporación Universitaria Lasallista, 2013-09-26) Franco Anaya, Piedad Astrith; Ramírez Medina, Luz Marcela; Orozco Ugarriza, Mauricio Ernesto; López Gutiérrez, Lersy AnaIntroduction. Pork meat is a very well regarded food, with high protein content and a good price. It is highly consumed in Cartagena, and this easy commercialization eases that in some cases the meat is not kept and sold under the most strict hygiene conditions. Keeping this meat under inadequate conditions brings infectious agents and an exaggerated number of microorganisms that create a public health problem. Objective. Determine E. coli and identify the O157:H7 serotype in pork meat commercialized in Cartagena´s supermarkets during August and September, 2008. Materials and methods. This research work used a quantitative approach with a descriptive cross-sectional type and a MPN (most probable number) technique according to INVIMA´s standards, and the Reveal device was used to identify the O157:H7 serotype. 60 samples of pork meat commercialized in 20 supermarkets in Cartagena were taken. 3 samples were taken in each supermarket, corresponding to three different parts of the animal (lean-rib-chop) in order to have a total of 60 samples. Results. E.coli was found in 36 samples, in unacceptable quantities corresponding to a 60%, and the O157:H7 serotype was found in 17 samples, corresponding to a 28%. Conclusion. 60% of the samples contaminated with E. coli and 28% of the samples that turned out to be positive for the O157:H7 serotype, are not considered as safe for human consumption. This demonstrates that there are deficiencies in the microbial quality of the food commercialized in supermarkets located in Cartagena, thus jeopardizing public health.