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Browsing Capítulos de libros by Subject "Bacterias ácido lácticas"

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    Evaluación de la adherencia de bacterias ácido-lácticas al epitelio intestinal de pollos alimentados con probióticos
    (Corporación Universitaria Lasallista, 2011-06-03) Gutiérrez Ramírez, Luz Adriana; Ramírez Arias, Luz Adriana
    Abastract: Introduction. The consumption of probiotics have a positive influence in the host organism’s health and increases its productivity, sensitiveness to pathogens and alimentary conversion, among other benefits. Objective. To identify, by the use of microbiological and molecular techniques, the adhesion of native bacteria with probiotic activity in the diet given to broilers. Methodology. 103 chickens, divided into three groups, were used. One of them was taken as a control. The second was fed with concentrated food without growth boosting antibiotics, and with probiotic bacteria, plus yeast. The third group was fed with concentrated food without growth boosters, but with commercial probiotics (Prokura Poll-S®). A chicken from every group was slaughtered in three moments (days 10, 20 and 42), and the cecum of each chicken was extracted and diluted until10-2, inoculating 0,1ml in MRS agar at 37ºC/anaerobically/72 hours. After this period, colony forming units (CFU) and identification by Gram were determined. For the molecular evaluation, DNA was extracted with a QIAGEN kit and by amplifying the 16s region of ribosomalDNA by PCR in order to identify the bacteria attached. Results. It was determined that the CFU account augments throughout time. In the last count, 352 CFU were found in the first group; 1604 in the second and 1260 in the third, corresponding to Lactobacillus, and the bacterial DNA was obtained, but the rDNA did not get amplified. Conclusions: An increase of CFU in the counts evaluated could be seen, but the bacteria species were not molecularly determined because more sensitive techniques are required to study them.