Histochemistry distribution of mucins and number of leucocytes in the lungs of postweaning pigs exposed to E. coli lipopolysaccharide (LPS)
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Abstract
Introduction. Currently, little is known about the effect of lipopolysaccharides (LPS)
derived from E. coli on the number of goblet cells and the type of mucins secreted
in the airways ofpiglets. Likewise, the differential distribution of these mucins and
their relationship with the development of respiratory infections in the post-weaning
period is not well understood. this information is useful to porcine production for
the development of preventive and sanitary management strategies. Objective. This
study aimed to evaluate the effect of the oral intake of E.coli LPS on the mucins
distribution and the number of leukocytes in the lungs of early weaned piglets.
Methods. 52 piglets weaned at 21 days were used. Animals were fed a basal diet
with four levels of LPS (0.0, 0.3, 0.5, and 1.0µg /mg feed) for 10 days.Pigs were
sequentially slaughtered on days 1, 5, 7, and 10 after weaning. Lung samples were
taken for histochemical stainings (H-E, Alcian blue pH 1.0, 2.5, and PAS) in order to
determine the amount and type of leukocytes as well as the number of goblet cells
and their type of mucins produced. Mucins were classified according to their dyeing
characteristicsas: acidic sulfated, non-sulfated, and neutral, all by computerized
image analysis.The statistical design used was a randomized blocks in a 4x4 factorial
arrangement (four experimental diets and four days post-weaning). Results and
discussion.Results showed a decrease in the number of goblet cells and neutral
mucins in both bronchi and bronchioles in the control piglets, followed by a recovery
inday 10 post-weaning. In groups with LPS such recovery was inhibited. In all study
groups acidic mucins increased in bronchi on day five. There were no acidic mucins
in their bronchioles. Moreover, the treatment with the higher concentration of LPS
caused a decrease in the number of neutrophils on day 10 and had no effects on
the amount of other leukocytes.Conclusions. LPS affects the recovery of goblet cells
in the lung airway epithelium and decreases the number of neutrophils altering the innate immunity in early weaning. Consequently, it may facilitate the entry of pathogens and an
allergen sensitization.
Description
Keywords
Corporación Universitaria Lasallista, Facultad de Ciencias Administrativas y Agropecuarias, Pulmones, Neutrófilos, Mucinas, Destete, Cerdos, Lipopolisacáridos
Citation
Revista Journal of Agriculture and Animal Sciences Vol. 6 N. 1