Pre-tratamiento de aguas residuales de la industria láctea con una lipasa inmovilizada
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2013
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Corporación Universitaria Lasallista
Área Metropolitana del Valle de Aburrá
Área Metropolitana del Valle de Aburrá
Resumen
Introduction. Waste water from dairy industries has a high fat content. This fat, due to its slow degradation,
makes its biological treatment difficult and brings operational problems Objective. To evaluate the efficiency
of an immobilized lipase from Candida rugosa in an organoclay, for the treatment of waste waters from a dairy
industry. materials and methods. An immobilized lipase from Candida rugosa was used to hydrolyze the fat
contained in a sample of waste water from the process of making cheese. The Michaelis-Menten (Km) cons-tant and the maximum reaction speed (Vmax) were obtained through nonlinear regression. The level of total
fat content reduction and the chemical oxygen demand (COD) were determined as results of the enzymatic
pre-treatment. Finally, the operational stability of the bio catalytic system was evaluated by the reuse of the
immobilized enzyme in three reaction cycles. Results. Km values were 421,71 ± 40,54 and 1513,79 ± 239,03
ppm, while Vmax were 10,74±1,03 and 0,30±0,05 μmol/mL*min for the free and the immobilized enzyme, resectively. A 70% reduction of total fat and 19, 20% of the COD were obtained after the enzymatic hydrolysis. The
fat reduction remained close to 67% after three reaction cycles using the immobilized lipase. Conclusion. The
results obtained demonstrated the potential of using immobilized lipases in the pre-treatment of waste water
with a high fat content.
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Corporación Universitaria Lasallista, Área Metropolitana del Valle de Aburrá, Industria láctea, Contaminación industrial, Aguas residuales, Aguas residuales industriales, Tratamiento de aguas residuales