Criopreservación de semen canino por congelación rápida por dimetilformamida
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Abstract
Introduction. Several factors influence the fertilizing
capacity of cryopreserved canine semen. Structural alterations and the osmotic and toxic effects on
sperm have focused interest on developing new cryopreservation and cryoprotection techniques with a
bigger potential for conserving the semen´s fertilization capacity. Objective. To compare the effect of
fast freezing with glycerol and dimethylformamide
on the post-freezing quality of canine semen. Materials and methods. In a cryopreservation process
by fast freezing, four treatments were implemented
(glycerol at 3% and 5%, y dimethylformamide at 3%
and 5%). After thawing, individual mobility, sperm
morphology and the membrane´s integrity of sperm
were evaluated. The statistic evaluation was made
by the use of a variance analysis and a Fisher´s minimal significant difference test. Results. For the individual mobility, a superiority was found for the 5%
glycerol (58%±7.8) over 5% DMF (44.5%±17.7), 3%
glycerol (18%±10.1), and 3% DMF (11.8%±10.5).
For the membrane´s integrity, 5%DMF had the highest media value (33.4%±9.7), above 3% glycerol
(27.5%±4.3) y 3% DMF (24.2%±5.3), but it had no
statistic difference over 5% glycerol (31.4%±3.8).
For the sperm morphology, no statistic difference
was found between 5% glycerol (67%±11.9), 3%
glycerol (65.6±12.5), and 5%DMF (60%±16.5), but
there was a difference between these and 3% DMF
(50.1%±18.3). Conclusions. Glycerol and DMF, in
5% concentrations, provide a similar protection to
semen during fast freezing cryopreservation.
Description
Keywords
Criopreservación, Corporación Universitaria Lasallista, Crioprotectores, Semen canino, Semen - Criopreservación, Glicerol, Dimetilformamida