Efecto de la Dimetilformamida sobre la viabilidad posvitrificación de embriones bovinos producidos in vitro
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Abstract
Introduction. Great efforts have been made in order
to improve the cryopreservation of embryos. Nevertheless,
one of the main problems is the sensible
they are to cryopreservation, due to the bad effects
of low temperatures and the concentration of cryoprotectors.
Given this, the use of combinations of cryoprotectors
has been proposed in order to reduce
the harm produced by cryopreservation. Objective.
To evaluate the effect of dymethylformamide (DMF)
on the post-vitrification feasibility of in vitro bovine
embryos. Materials and methods. 123 in vitro
produced and vitrified in open pulled straws (OPS)
embryos were randomly distributed according to the
study groups (T1: DMSO + DMF 15%, T2: DMSO +
DMF 20% and control: EG + DMSO 20%). After the
de-vitrification, the embryos were cultivated and the
re-expansion percentage was determined at 6 hours
and its maintenance, at 18 hours of cultivation as a
parameter of embryonic feasibility. Results. When
the re-expansion of vitrified embryos in the T1, T2
and control were determined at 6 hours (61,9%,
91,6% and 63,8%) and at 18 hours (83,3%, 91,6%
and 63,8%), the highest re-expansion percentages
were found in the combinations with DMF. Conclusion.
The combination of the 20% DMSO cryoprotectors
+20% DMF during the vitrification of in vitro
produced bovine embryos + 20% DMF during the
vitrification of the bovine embryos produced, keeps
the morphology and the capacity to re-expand or recover
the blastocoel, after the thawing.
Description
Keywords
Corporación Universitaria Lasallista, In vitro, Dimetilformamida, Ganado - Reproducción, Criopreservación, Embriones bovinos, Vitrificación