Producción de lacasa utilizando Pleurotus ostreatus sobre cáscaras de plátano y bagazo de caña
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2010
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Corporación Universitaria Lasallista
Resumen
Introduction. Plantain peel for industrial purposes
represents from 35% to 40% of the total fruit weight,
becoming a considerable quantity of waste which
could be used to produce enzymatic complexes. Objective. To investigate the potential green and mature plantain peel of “Dominico-Hartón” as a supportsubstrate to produce laccase extracellular enzyme
by the use of Pleurotus ostreatus fungus. Materials
and Methods. Pleurotus ostreatus fungus was cultivated in different mixtures, as follows: Green plantain peel (GPP), mature plantain peel (MPP) green
plantain peel-cane bagasse (50/50) (GPPCB), mature plantain peel-cane bagasse (50/50) MPPCB).
Fermentation units were kept at pH 4.8, 28°C and a
63% humidity. The determination of the laccase enzyme was carried out by ABTS oxidation. Results.
In the substrates used, a maximum laccase activity
was achieved in the 24
th
day, except in the mature plantain treatment, which achieved it in the 34
th
day. MPPCB substrate had the highest production of
laccase with 19.4 U/mL concentration. Conlcusion.
Plantain peel can be a good substrate when is supplemented with other lignocellulosic wastes, such
as sugar cane bagasse. Laccase can be used as a
morphogenetic indicator because it shows the maximum growth of the mycelium.
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Palabras clave
Bagazo de caña, Pleurotus ostreatus, Dominico - Hartó, Plátano dominico - Hartó, Enzima extracelular lacasa, Cascara de plátano, Residuos sólidos agrícolas, Corporación Universitaria Lasallista