Efecto de la aplicación de dexametasona en la evolución de la enfermedad hepática crónica, en un modelo de experimentación murino
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Fecha
2011
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Corporación Universitaria Lasallista
Resumen
Introduction. Liver cirrhosis is one of the biggest
public health problems Worldwide. There have been
great advances in learning about the molecular aspects of this disease. The detailed knowledge of such
mechanism would be the basis of research about
the therapeutic possibilities in the reversal of fibrogenesis. Objective. To Determine the effect of the
therapy with dexomethasone on fibrogenesis and on
the liver´s function in a cirrhosis murine experimental
model. Materials and method. 36 Wistar rats were
used. They came from the Universidad de Caldas´
vivarium and they were given cirrhosis with carbon
tetrachloride (CCl4). The project was supported
by the Universidad de Caldas´ Ethics Commission for Research with Animals. Two treatments were
taken (T1=CCl4 and T2=CCl4 + dexamethasone).
Samples were taken every two weeks for histopathology and plasma albumin measurement. Those
treatments were distributed in an Unrestricted Random Design, made up by 2 treatments, 5 repetitions
and three animals per experimental unit and a control group (n=6). A variance analysis (ANOVA) was
performed, fixing a p≤0.05 and the Turkey´s multiple
comparison test was performed when a statistically
significant difference appeared. Results. Significant
differences were not observed in the fibrogenesis,
leukocyte infiltration and vacuolar degeneration between treatments. In T2, reduced food consumption
and a body weight loss statistically inferior to that
of T1 were observed. Conclusion. The application
of dexamethasone kept a superior plasma albumin
level, which possibly means a better function of the
liver. No differences were observed in the fibrogenesis progression with this treatment.
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Palabras clave
Cirrosis, Corporación Universitaria Lasallista, Enfermedades hepáticas (Medicina Veterinaria), Modelo de experimentación murino, Cirrosis hepática, Albúmina, Dexametasona, Fibrogénesis