Biodisponibilidad de Trichoderma asperellumen el tiempo en suelo Andisol en condiciones de laboratorio y campo
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Date
2015-08-24
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Abstract
Introduction. Trichoderma asperellum strain T109
has shown great antagonism activity in vitro against
phytopathogenic fungi. However, it is necessary the
real understand of this strain into spatial organization
in soil under in vitro and field conditions to know
the population density. Objective. The aim of this
study was to evaluate different inoculum concentrations
and frequencies of application in Trichoderma
asperellum T109 bioavailability. Materials and
methods. Spore suspensions containing 1x106 and
1x108 conidia.mL-1, were evaluated under controlled
laboratory and field conditions, with different application
times. We used the technique of Petri plate
count for determination of CFU.g-1 of soil. Results.
The T109 bioavailability was affected by the frequency
of application and highly significant (p<0.001) for
the initial concentration of inoculum. The last sampling
under in vitro conditions showed that the concentration
of 1x106 conidia mL-1 was significantly
different (p<0.001) when compared to 1x108 conidias.
mL-1, yielding maximum value of CFU.g-1 of soil
for every 4 days of application. For field conditions,
we obtained significant differences (p<0.001) when
the concentration of 1x108conidias mL-1 and application
of 7 days were tested concomitantly. Conclusions.
We can determine that the population density
of T. asperellum T109 is important in the establishment
of the fungus in the soil.
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Keywords
Corporación Universitaria Lasallista, Trichoderma asperellum, In vitro, Densidad de población