Evaluación de dos diluyentes para la criopreservación de semen de caballos de la raza criollo colombiano
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2014
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Corporación Universitaria Lasallista
Resumen
Introduction. The composition of the extenders
for cryopreservation of stallion semen is crucial in
cryotolerance and post-thaw fertilizing capacity of
sperm cells. Objective. Evaluate two extenders for
freezing the semen of Colombian creole stallions.
Materials and methods. Semen of four Colombian
creole stallions was cryopreserved by fast freezing
protocol with Equipro® and Gent® extenders. Evaluation of sperm post-thaw motility was performed
by a Sperm Class Analyzer system (SCA®) to examine total motility (TM), progressive motility (PM),
velocity curvilinear (VCL), velocity straight line (VSL)
and velocity average path (VAP). The sperm vitality
(SV) and normal morphology (NM) were assessed
by eosin-nigrosin staining; the plasmatic membrane integrity was determined by the hypo-osmotic
swelling test (HOS). For statistical evaluation were
adjusted generalized linear models (GLM) and the
treatments averages were compared by the Tukey
test. Associations between variables were assessed
by Pearson correlation analysis. Results. Statistical
difference (P≤ 0.05) was found for TM with averages of 50.9% ± 19.4% and 42.2% ± 15.1% for Equipro® and Gent®, respectively. Except for VSL no
difference was found between the other evaluated
parameters. There was a high association between
motility parameters, vitality and membrane integrity.
Conclusions. Equipro® extender produces superior
post-thaw total motility rates than Gent® extender in
sperm of Colombian creole stallions.
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Corporación Universitaria Lasallista, Caballo Criollo Colombiano, Caballos - Mejoramiento de la especie, Caballos - Reproducción, Biotecnología reproductiva