Determinación de cadmio en leches crudas usando un biosensor amperométrico
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Date
2012
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Abstract
Introduction. Cadmium is a metal that has been
found in milks and, therefore, represents a big risk for
human health. Biosensors have been used as tools
for the rapid determination of this kind of compounds
in several matrices. Materials and methods. An amperometric
biosensor and screen printed electrodes
were used to immobilize the urease enzyme, by the
use of the cross linking method. The subtract used
for the determination was urea. Before determining
the cadmium concentration in the milk, the method
used was validated by making calibration curves
and evaluating the repeatability of both the method
and the instrument. Results and discussion. The
linear rank for the method was 5 mM to 60 mM with
a high correlation between variables. The quantification
limit of the method was 0.05 mg/L. The variation
coefficient was 7.55 % for the method´s repeatability
tests, which indicates a high precision of the
results. From the milk samples analyzed, recovery
percentages above 80% were obtained. This means
a low matrix effect, keeping in mind that milk has a
high degree of interfering elements for this kind of
methods. Conclusion. The amperometric biosensor
demonstrated to be an efficient and fast tool to determine
cadmium in milks, and this can be helpful to
improve the milk´s quality in swine farms.
Description
Keywords
Corporación Universitaria Lasallista, Biosensores, Leche, Leche - Análisis, Leche - Calidad, Leche - Contaminación, Cadmio, Contaminación por cadmio