Efecto de la osmolaridad, sobre el diámetro y la calidad de oocitos bovinos madurados in vitro
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Date
2009
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Abstract
Ooocytes are susceptible to osmolar changes, and
show some phenomena that affect the plasma membrane
Objective. To evaluate the effect of hypo and
hyperosmolarity from the culture’s environment on
the diameter and the quality of in vitro matured oocytes.
Materials and methods. 75 oocytes of excellent
and good quality were use in the study. As a control
the M 199 was used, suplemented with 10 % of
FCS, 30 μg of FSH and 30 μg of LH (290 mOsm/l
T2). The oocytes were evaluated under two osmolar
concentrations: Hyperosmotic media, 497mOsm/L
(T1), and hyposmotic media 150mOsm/L (T3). The
diameter and the quality were measured at 0 hours
and after 24 hours. Results. The oocyte diameter at
24 hours was bigger in the T3 (148,7±9.7) and smaller
in the T1 (134,6±1.2). Regarding the control
(147.9±11.5) (p <0.05.), no difference was found between
the percentage of excellent and good quality
oocytes after 24 hours of treatment T1 in comparison
to the control (56 % and 60 %) (p> 0.05.), but the
quality in the T3 was lower (36 %). Conclusions. It
was demonstrated that the hyperosmotic media had
a bigger detrimental effect on the oocytes than the
hyposmotic media. It is possible that the oocytes show
more mechanisms to avoid the water flow into the
cytoplasm than to avoid the exit of solutes and water
flow towards the outside. The necessity to adopt rigorous
measurements in the quality control of osmolarity
for the culture mediums used in the processes
of in vitro maturation for oocytes, is evident.
Description
Keywords
Membrana celular, Oocitos bovinos, Hipertónico, Hipotónico, Membrana celular, Oocito, In vitro, Osmolaridad