Producción limpia de conservantes orgánicos para la industria de alimentos obtenidos a partir de la extracción de componentes bio-activos de la semilla del algarrobo (Hymenaea courbaril Linneaus), utilizando las tecnologías de fluidos supercríticos y Soxhlet
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Date
2012-07-17
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Área Metropolitana del Valle de Aburrá
Área Metropolitana del Valle de Aburrá
Abstract
Introduction. Carob (Hymenaea courbaril Linneaus) is a plant known because of its several
antifungal, molluscicide, nutritional and protective properties, all of which can be applied in
food industries.
Objective. This research work is made extracting the bioactive components of carob seeds,
by the use of Soxhlet with polar and non- polar solvents and supercritical fluids with carbon
dioxide (FSC-CO2), to evaluate its antimicrobial properties on Escherichia coli ATCC 25922 and
determine the performance of every extraction.
Materials and methods. The carob seeds were smashed and characterized before the
respective tests. Then, the performances of the inhibitory component extracted were compared.
The extraction was made by the use of two methods: Soxhlet and supercritical fluids, to
determine the antimicrobial action spectrum at different concentrations (%v/v) (100%, 50%,
10%).To study the data obtained, the one way variance analysis method (ANOVA) was used
for the performance response variable (%) and the two ways method for the microbiological
analysis variable. Results. The performance analysis (%) had significant differences (p<0.05) between each
extract obtained for the microbiological analysis (length of inhibition halo in cm). The same
happened (p<0.05) between extracts and for the concentrations evaluated.
Conclusion. The bioactive compounds of the carob seed have an antimicrobial activity on E.
Coli. These compounds are soluble in ethanol and their performance is optimized by a previous
extraction by the use of a cleaner production technology, like FSC-CO2.
Description
Keywords
Algarrobo (hymenaea courbaril linneaus), Corporación Universitaria Lasallista, Área Metropolitana del Valle de Aburrá, Fluido supercrítico, Aditivos (alimentos), Componentes bio-activos, Extractor soxhlet, Industria alimenticia