Comparación del método de Friedewald y el directo para la determinación del colesterol LDL en gallinas ponedoras
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Date
2015-08-24
Journal Title
Journal ISSN
Volume Title
Publisher
Corporación Universitaria Lasallista
Abstract
Introduction. The methods to evaluate blood lipids
are varied in the lipid profiles of poultry. In this paper
two methods to quantify the cholesterol of low density
lipoproteins in laying hens are compared. Materials
and methods. The low density lipoproteins´
cholesterol was quantified by the use of the
Fredewald´s method and of the direct method based
on detergents and N, N Bis (4-sulfobutyl) -m-toluidine
in 40 laying hens from the Hy-Line W-36 line, with
26 weeks of age. Results. There are differences
between methods in the results.The P value of test
F is below 0,05, showing a statistically significant
difference, with a 95 % confidence between methods.
Conclusions. The use of the direct method based on
detergents and N, N Bis (4-sulfobutyl) -m-toluidine to
determine the low density lipoproteins´ cholesterol in
laying hens is feasible.
Description
Keywords
Corporación Universitaria Lasallista, Colesterol, Gallinas ponedoras, Colesterol - Metabolismo