(Corporación Universitaria Lasallista, 2012-08-21) Giraldo Giraldo, John Jairo; Ordóñez Ramírez, Sebastián; Álvarez Arango, Andrea
Cryopreservation of bovine embryos produced in vitro eases the use of embryo
transfer programs, establishing germplasm banks with a permanent access to genetic
material of a determined individual or race and the biotechnologies related such as
cloning and transgenesis (Albarracín, 2005). Therefore, this has brought a necessity
to conserve the embryos remaining from the in vitro production. Nevertheless, the
cryopreservation of such embryos by the use of conventional freezing methods has not reached satisfactory survival rates (Vajta, 2000),
so other methods, such as vitrification, have been
being used. This method hast cooling curves
above those from the traditional freezing, allows
to reduce the embryo´s exposition time in the
critical temperature points, reduces thermal and
mechanic damage caused during the formation
of ice and increases the feasibility of the embryos
after the devitrification (Lazar, 2000; Arteaga et
al., 2002; Vajta and Kuwayama, 2006, Mucci et
al., 2006).
