Browsing by Author "Giraldo Giraldo, John Jairo"
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Item Buenas prácticas ganaderas en la rutina de ordeño mecánico en la Hacienda Primavera(Corporación Universitaria Lasallista, 2014) Agudelo Carmona, Juan Pablo; Giraldo Giraldo, John JairoItem Efecto de la Dimetilformamida sobre la viabilidad posvitrificación de embriones bovinos producidos in vitro(Corporación Universitaria Lasallista, 2012) Giraldo Giraldo, John Jairo; Gómez Oquendo, Jorge; Vásquez Araque, NeilIntroduction. Great efforts have been made in order to improve the cryopreservation of embryos. Nevertheless, one of the main problems is the sensible they are to cryopreservation, due to the bad effects of low temperatures and the concentration of cryoprotectors. Given this, the use of combinations of cryoprotectors has been proposed in order to reduce the harm produced by cryopreservation. Objective. To evaluate the effect of dymethylformamide (DMF) on the post-vitrification feasibility of in vitro bovine embryos. Materials and methods. 123 in vitro produced and vitrified in open pulled straws (OPS) embryos were randomly distributed according to the study groups (T1: DMSO + DMF 15%, T2: DMSO + DMF 20% and control: EG + DMSO 20%). After the de-vitrification, the embryos were cultivated and the re-expansion percentage was determined at 6 hours and its maintenance, at 18 hours of cultivation as a parameter of embryonic feasibility. Results. When the re-expansion of vitrified embryos in the T1, T2 and control were determined at 6 hours (61,9%, 91,6% and 63,8%) and at 18 hours (83,3%, 91,6% and 63,8%), the highest re-expansion percentages were found in the combinations with DMF. Conclusion. The combination of the 20% DMSO cryoprotectors +20% DMF during the vitrification of in vitro produced bovine embryos + 20% DMF during the vitrification of the bovine embryos produced, keeps the morphology and the capacity to re-expand or recover the blastocoel, after the thawing.Item Efecto de los suplementos fluido folicular y suero fetal bovino sobre la maduración in vitro de oocitos equinos(Corporación Universitaria Lasallista, 2011-06-03) Restrepo Betancur, Giovanni; Giraldo Giraldo, John Jairo; Gómez Oquendo, Jorge; Vásquez Araque, Neil; Vásquez Correa, Eliana María; Ortega Flórez, Jorge Andrés; Córdoba, Luís FernandoAbastract: Introduction. The production of embryos in horses is a technique that provides higher reproduction efficiency for animals with a high genetic value. The access to it and its success are limited, though, due to the difficulties to reach efficient fertilization rates by the use of in vitro conventional fertilization, and given the need of many technical and economic resources for ICSI (intracytoplasmic sperm injection), among other limitations for embryo development in vitro. A fundamental step for embryo production in horses is the in vitro maturation, because it determines the capability an egg has to become an embryo. Several media, fluids and molecules have been evaluated, aiming to improve this process, but an optimal composition of the maturation medium for horse oocytes is not yet known. Objective. To evaluate two components for in vitro maturation: The bovine fetal serum and the horse’s follicular fluid, to understand the physiological reproductive conditions of mares in our environment and what component is better for the maturation as an indicator of the oocyte´s ability to develop itself as an embryo. Materials y Methods. For the in vitro maturation, the bovine fetal serum and the horse´s follicular fluid, they were individually included in a 20% proportion in TCM-199 medium, additionally supplemented with gonadotropins (FSH and LH), antibiotics, glutamine, insulin, transferrin and selenium. Groups of 5 oocytes were incubated during a 36 hours period in controlled environmental conditions, at 39°C, 5% of CO2 and 90% of relative humidity. Results. Higher percentages of maximum expansion of the cluster could be seen for oocytes supple mented with bovine fetal serum (47.5%) compared with those maturated with horse’s follicular fluid (16,2%). No statistic difference was found for the in vitro maturation percentages between treatments. Conclusion. The bovine fetal serum as a supplement for in vitro maturation has a beneficial effect on the cluster expansion of horse’s oocytes.Item Efecto del nivel de suplementación con propilenglicol durante el período de transición a la lactancia sobre actividad ovárica y desempeño reproductivo en vacas Holstein(Corporación Universitaria Lasallista, 2017) Castro Ruiz, Sandra Marcela; Galvis Góez, Rubén Darío; López Herrera, Albeiro; Giraldo Giraldo, John JairoIntroduction. This paper mainly focuses on the study of the inclusion of propylene glycol (PG) as energy compound and its possible effect on minimizing the negative energy balance. Objetive. Evaluate on reproductive parameters, specifically on rates of calving to first service, days open, calving to first observed heat, as well as its effect on insulin secretion and ovarian activity. Materials and Methods. 20 Holstein cows were assigned to one of four supplementation levels: 0, 300, 500 and 700 grams of PG / day mixed in food, from day 10 prepartum and through day 20 postpartum. Blood samples were collected on 10 and 5 prepartum and days 0, 5, 10 15 and 20 postpartum to determine the concentration of insulin were also done to determine transrectal ultrasound follicular growth and ovarian resumption. All data for the evaluation of reproductive parameters were taken from the day of delivery, making daily observations of animals 30 minutes in the morning and 30 minutes in the afternoon to record possible heat presented. Results. There were no significant differences for the variables within the different treatments (p> 0.05), only a trend to decrease the open days when you include a dose of 500 g of propylene glycol with respect to the other groups. Conclusions. The inclusion of propylene glycol had no significant effect on insulin secretion, or the number and size of preovulatory follicles (p> 0.05). It is very likely that the propylene supply mode and time of collection of blood samples affect the idItem Evaluación de la estimulación ovárica y la calidad de oocitos bovinos obtenidos por aspiración folicular(Corporación Universitaria Lasallista, 2017) Giraldo Giraldo, John Jairo; Ordoñez Ramirez, Sebastián; Gómez Oquendo, Jorge; Restrepo Betancur, GiovanniIntroduction. Ultrasound-guided follicular aspiration (UGFA) coupled with the in-vitro production of embryos is an important alternative for the use of genetic resources of bovine females of high productive potential. However, the reduced quality of oocytes recovered with this technique is one of the aspects with the highest impact on the efficiency to obtain embryos. Materials and methods. This research compared follicular development with the quality of oocytes recovered by UGFA, in bovine females conventionally over-stimulated with FSH or FSH and hCG. Results. A higher quality of oocytes recovered from animals stimulated with FSH and hCG was seen compared to the ones stimulated with FSH only. Conclusions. Inclusion of Hcg in a scheme for the ovarian over-stimulation with FSH of bovine females subject to follicular aspiration becomes a favorable effect on the quality of recovered oocytes.Item Evaluación de la relación del tamaño del folículo dominante al momento de la inseminación artificial con la tasa de concepción en vacas Holstein de alta producción.(Corporación Universitaria Lasallista, 2015) Álvarez Arango, Andrea; Giraldo Giraldo, John JairoItem Evaluar efecto de dos protocolos de inseminación artificial en hembras porcinas sobre porcentaje de natalidad y su relación costo beneficio.(Corporación Universitaria Lasallista, 2015) Mora Echavarría, Juliana; Aguilar González, Manuela; Giraldo Giraldo, John JairoItem Implementación de registros productivos y reproductivos en tres unidades ganaderas del departamento de Córdoba.(Corporación Universitaria Lasallista, 2015) Londoño Correa, Camila Andrea; Giraldo Giraldo, John JairoItem Implementación del control del rendimiento lechero en lecherías del altiplano norte de Antioquia(Corporación Universitaria Lasallista, 2013) Quijano Caro, Luis Fernando; Giraldo Giraldo, John JairoItem La vitrificación como alternativa de conservación de embriones producidos in vitro(Corporación Universitaria Lasallista, 2012-08-21) Giraldo Giraldo, John Jairo; Ordóñez Ramírez, Sebastián; Álvarez Arango, AndreaCryopreservation of bovine embryos produced in vitro eases the use of embryo transfer programs, establishing germplasm banks with a permanent access to genetic material of a determined individual or race and the biotechnologies related such as cloning and transgenesis (Albarracín, 2005). Therefore, this has brought a necessity to conserve the embryos remaining from the in vitro production. Nevertheless, the cryopreservation of such embryos by the use of conventional freezing methods has not reached satisfactory survival rates (Vajta, 2000), so other methods, such as vitrification, have been being used. This method hast cooling curves above those from the traditional freezing, allows to reduce the embryo´s exposition time in the critical temperature points, reduces thermal and mechanic damage caused during the formation of ice and increases the feasibility of the embryos after the devitrification (Lazar, 2000; Arteaga et al., 2002; Vajta and Kuwayama, 2006, Mucci et al., 2006).Item Relación del tamaño del folículo dominante y tasa de concepción al momento del servicio en vacas holstein de alta producción(Corporación Universitaria Lasallista, 2015-09-03) Álvarez Arango, Andrea; Giraldo Giraldo, John JairoIntroducción. According to the research conducted, some correlations between the dominant follicle´s size and the fertility in high producing cows have been reported. However, there are still some knowledge gaps concerning the effect of follicular dynamics on the conception. Objective. To identify the correlation between the dominant follicle size at the artificial insemination time and the conception rates in high producing Holstein cows. Material and methods. 42 high production animals were evaluated, determining their dominant follicle´s size at the artificial insemination time and location (right or left ovaries), body condition score (BCS) and body weight. The data were analyzed by means of the Statgraphics software, version 15; a confidence level of 95% was used to find significant differences. By adjusting regression equations, the relationships between the size of the dominant follicle at the time of service and the conception rate with the size of the dominant follicle were established. Results. A connection between the three average sizes of the follicles related to the body condition and the effective service with each one of the weights was found. As the dominant follicle´s size increased, so did the effective service percentages but, when the weights and the body conditions of the animals increased, the effective service percentages decreased. The larger the dominant follicle size, the higher the effective service percentage. However, if the animal’s weight and body conditions increase, the effective service percentage decreases. Conclusions. At the service time, the follicular diameter is directly related to the rate of conception, regardless of the ovary in which the dominant follicle is. This fact could be considered as a fertility indicator.Item Sincronización y resincronización de celos y de ovulaciones en ganado de leche y carne(Corporación Universitaria Lasallista, 2008) Giraldo Giraldo, John JairoIn the dairy cattle the detection of cows in state of zeal has become one of the most important factors that diminish the reproductive efficiency. Although diverse complementary methods exist to improve the detection of those cows, the synchronization of ovulations and systematic insemination of all of the animals which state of zeal has not been detected, has become a viable and easy alternative of implementing with that from which a fertility rate from 35 to 40% can be obtained. In meat herds, artificial insemination has not been used as a tool for the genetic improvement, due to the difficulty in detecting the animals in zeal, and this is due to the geography of the pasture grounds and to the technical capacity of the personnel in charge of detecting animals in zeal, and of inseminating them. The implementation of programs for synchronizing ovulations and systematic insemination allows the elimination of the zeal detection process in breeding herds and concentrate the efforts on the artificial insemination task in a short time, allowing this task to be made by the veterinarian or a qualified technician and achieving a fertility rate from 40 to 50%. The combination of programs of ovulation resynchronizations with the precocious detection of empty animals, allows the systematic insemination of the empty animals without detecting zeal in the returns and to achieve a fertility rate from 55 to 75%, in two insemination rounds. In summary, the resynchronization ovulation and systematic insemination programs allow the elimination of the zeal detection in dairy cattle and beef cows, and also helps to maintain a level of viable fertility.Item Una mirada al uso de la inseminación artificial en bovinos(Corporación Universitaria Lasallista, 2007) Giraldo Giraldo, John JairoWe aim to analyze the influence that this biotechnology has had from its introduction into our country, Colombia, in fundamental aspects for the bovine exploitation as the reproductive efficiency, the genetic improvement, the technology transfer to the country and the training of the producer, everything finally reflected in the productivity and the profitability of the cattle exploitations.